Down regulation of intimin expression during attaching and effacing enteropathogenic Escherichia coli adhesion
نویسندگان
چکیده
منابع مشابه
Genetic diversity of intimin genes of attaching and effacing Escherichia coli strains.
In this study, we determined the sequences of four intimin variant genes detected in attaching and effacing Escherichia coli isolates of human origin. Three of them were novel and were designated eae-eta (eta), eae-iota (iota), and eae-kappa (kappa). The fourth was identical to the recently described eae-zeta (zeta), isolated from a bovine E. coli O84:NM isolate. We compared these sequences wit...
متن کاملTyping of intimin (eae) genes in attaching and effacing Escherichia coli strains from monkeys.
Attaching and effacing Escherichia coli (AEEC) strains cause histopathological alterations termed “attaching and effacing (A/E) lesions” (8). The ability to cause A/E lesions is encoded on a large bacterial chromosomal pathogenicity island, the locus of enterocyte effacement (LEE). The central portion of LEE encodes intimin (Eae, 94to 97-kDa outer membrane protein) and Tir, the intimin receptor...
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The cytoskeletal lesions associated with enteropathogenic Escherichia coli adhering to cultured HeLa epithelial cells were examined by immunofluorescence microscopy. The microfilament-associated proteins actin, alpha-actinin, talin, and ezrin were localized with adherent enteropathogenic E. coli, whereas tropomyosin, keratin and vimentin (intermediate filaments), tubulin (microtubules), and vin...
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Attaching and effacing (AE) bacteria are a diverse group of gastrointestinal pathogens, comprising members of four genera, that cause the intestinal epithelial microvilli to be replaced with raised clusters of filamentous actin that conform to the surface of attached bacteria. We have cloned a 35.4 kb 'pathogenicity island' from the prototype AE bacterium, enteropathogenic Escherichia coli, con...
متن کاملInteraction of the enteropathogenic Escherichia coli protein, translocated intimin receptor (Tir), with focal adhesion proteins.
When enteropathogenic Escherichia coli (EPEC) attach and infect host cells, they induce a cytoskeletal rearrangement and the formation of cytoplasmic columns of actin filaments called pedestals. The attached EPEC and pedestals move over the surface of the host cell in an actin-dependent reaction [Sanger et al., 1996: Cell Motil Cytoskeleton 34:279-287]. The discovery that EPEC inserts the prote...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1997
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.65.5.1644-1652.1997